Chromomycin A3 staining, sperm chromatin structure assay and hyaluronic acid binding assay as predictors for assisted reproductive outcome
نویسندگان
چکیده
منابع مشابه
Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques.
The predictive value of sperm chromatin integrity for pregnancy outcome following in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) was studied in 24 men attending a university-based assisted reproductive techniques laboratory using the flow cytometric sperm chromatin structure assay (SCSA). The SCSA is a measure of the susceptibility of sperm DNA to low pH-induced denatu...
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Chromomycin A3 binds to DNA and Protamine -deficient spermatozoa look bright yellow,whereas normal spermatozoa appear dull yellow.
متن کاملRelation Between Sperm Binding Ability to Hyaluronic Acid With Protamine Deficiency and DNAFragmentation
Purpose: The best sperm selection by its binding ability to hyaluronic acid for ICSI and assessment of correlation between sperm binding ability to hyaluronic acid with sperm parameters, protamine deficiency and DNA fragmentation. Materials and Methods: Semen samples collected from 55 infertile couples referring to ?? Fertility and Infertility center were collected during this study. Semen ana...
متن کاملEvaluation of Protamine Level in Human Sperm Samples Using Chromomycin A3 and Aniline Blue Staining
Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...
متن کاملEtiology and Evaluation of Sperm Chromatin Anomalies
Evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. Routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear DNA integrity of spermatozoa. It has been suggested that altered nuclear chromatin struc...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2009
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2009.07.002